| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452404 |
| ISSN: | 0074-0276 |
| Authors: | Santos, Mayara Gil de Castro1 Trindade, Camilla Nunes dos Reis1 Vommaro, Rossiane Cláudia2 Domingues, Regina Maria Calvalcanti Pilotto1 Ferreira, Eliane de Oliveira1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Departamento de Microbiologia Medica, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease’s symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix. OBJECTIVE There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1). METHODS A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry. FINDINGS Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion. MAIN CONCLUSIONS These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Microbiología, Diarrea, Adhesión bacteriana, Laminina, Virulencia, Clostridioides difficile |
| Keyword: | Gastroenterology, Microbiology, Diarrhea, Bacterial adhesion, Laminin, Virulence, Clostridioides difficile |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
