Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO
Document title: Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO
Journal: Journal of applied research and technology
Database: PERIÓDICA
System number: 000427614
ISSN: 1665-6423
Authors: 1
1
1
1
1
1
2
Institutions: 1Universidad Autónoma Metropolitana, Departamento de Química, Iztapalapa, Ciudad de México. México
2Instituto Politécnico Nacional, Escuela Superior de Ingeniería Química e Industrias Extractivas, Ciudad de México. México
Year:
Season: Dic
Volumen: 16
Number: 6
Country: México
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm
Disciplines: Ingeniería,
Química
Keyword: Ingeniería química,
Ingeniería de materiales,
Biotecnología,
Inmovilización enzimática,
Lipasas,
Oxido de calcio,
Biomateriales
Keyword: Chemical engineering,
Materials engineering,
Biotechnology,
Enzyme immobilization,
Lipases,
Calcium oxide,
Biomaterials
Full text: Texto completo (Ver HTML) Texto completo (Ver PDF)