| Journal: | International braz j urol |
| Database: | PERIÓDICA |
| System number: | 000296842 |
| ISSN: | 1677-5538 |
| Authors: | Carvalho, Mauricio1 Lulich, Jody P2 Osborne, Carl A Nakagawa, Yasushi3 |
| Institutions: | 1Universidade Federal do Parana, Departamento de Medicina Interna, Curitiba, Parana. Brasil 2University of Minnesota, College of Veterinary Medicine, Saint Paul, Minnesota. Estados Unidos de América 3University of Chicago, School of Medicine, Chicago, Illinois. Estados Unidos de América |
| Year: | 2006 |
| Season: | May-Jun |
| Volumen: | 32 |
| Number: | 3 |
| Pages: | 342-349 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | INTRODUCTION: Nephrocalcin (NC) is a glycoprotein produced in the kidney and inhibits calcium oxalate crystal formation. It has been separated into 4 isoforms (A, B, C, and D) and found that (A + B) are more abundant than (C + D) in urine of healthy subjects, but the reverse is seen in human urine of kidney stone patients. To further examine the role of this protein in inhibition of urinary crystallization, nephrocalcin isoforms were purified from 2 genetically pure dog species. MATERIALS AND METHODS: We studied healthy Beagles, known to be non-stone forming dogs, and Mini-Schnauzers, known to be calcium oxalate stone formers. NC was isolated and purified from each group. Urinary biochemistry and calcium oxalate crystal growth inhibition were measured. RESULTS: Specific crystal growth inhibition activity was significantly higher in non-stone forming dogs (9.79 ± 2.25 in Beagles vs. 2.75 ± 1.34 of Mini-Schnauzers, p < 0.005). Dissociation constants toward calcium oxalate monohydrate were 10-fold different, with Beagles' isoforms being 10 times stronger inhibitors compare to those of Mini-Schnauzers'. Isoforms C + D of NC were the main isoforms isolated in stone-forming dogs. CONCLUSION: NC of these two species of dogs differently affects calcium oxalate crystallization and might have a role in determining ulterior urinary stone formation |
| Disciplines: | Medicina |
| Keyword: | Medicina experimental, Nefrología, Litiasis renal, Oxalato de calcio, Cristalización, Perros |
| Keyword: | Medicine, Experimental medicine, Nephrology, Renal lithiasis, Calcium oxalate, Crystallization, Dogs |
| Full text: | Texto completo (Ver HTML) |
