| Revista: | International braz j urol |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000434710 |
| ISSN: | 1677-5538 |
| Autores: | Li, Xue-Chao1 Huang, Chuan-Xi2 Wu, Shi-Kui1 Yu, Lan3 Zhou, Guang-Jian3 Chen, Li-Jun1 |
| Instituciones: | 1Chinese PLA General Hospital, The Fifth Medical Center, Beijing. China 2Hebei University, College of Life Science, Hebei. China 3Beijing Institute of Biotechnology, Laboratory of Medical Molecular Biology, Beijing. China |
| Año: | 2019 |
| Periodo: | Sep-Dic |
| Volumen: | 45 |
| Número: | 5 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Objective This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa. Materials and Methods A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the suppression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test. Results A cell line model with a low expression of filamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following: Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P <0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P <0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P <0.05). Conclusion The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene |
| Disciplinas: | Medicina |
| Palabras clave: | Medicina experimental, Oncología, Urología, Terapéutica, Neoplasias, Próstata, Filamina, ARN de interferencia, Genes supresores |
| Keyword: | Experimental medicine, Oncology, Urology, Therapeutics, Neoplasms, Prostate, Filamin, Interference RNA, Suppresor genes |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
