| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459252 |
| ISSN: | 1415-4757 |
| Authors: | Fan, Xiaojie1 Yang, Jingnan1 Wu, Guangyao1 Wang, Meiyi2 Cheng, Xiaoxia2 Liu, Chang2 Liu, Qian2 Wen, Yanan1 Meng, Shuangshuang1 Wang, Zhenxing1 Lin, Xuhong1 An, Lei1 |
| Institutions: | 1Huaihe Hospital of Henan University, Kaifeng, Henan. China 2Henan University, School of Medicine, Kaifeng, Henan. China |
| Year: | 2022 |
| Volumen: | 45 |
| Number: | 2 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ transfection reagent. The shRNA significantly decreased the expression of GFP. In addition, pre-diluted transfection reagent at room temperature and small nucleic acids increased the transfection efficiency, which peaked at 24 h. Compared with circular nucleic acids, linear nucleic acids showed higher transfection efficiency and a higher genome integration rate. We optimized cationic polymer-mediated RNAi conditions, and our data will be useful for future RNAi studies |
| Disciplines: | Biología, Medicina veterinaria y zootecnia |
| Keyword: | Genética, Bovinos, ARN, Polimerización, Cationes, Transfección |
| Keyword: | Genetics, Bovines, RNA, Transfection, Polymerization, Cations |
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