Optimization of cationic polymer-mediated transfection for RNA interference
Título del documento: Optimization of cationic polymer-mediated transfection for RNA interference
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459252
ISSN: 1415-4757
Autores: 1
1
1
2
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2
2
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1
1
1
Instituciones: 1Huaihe Hospital of Henan University, Kaifeng, Henan. China
2Henan University, School of Medicine, Kaifeng, Henan. China
Año:
Volumen: 45
Número: 2
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ transfection reagent. The shRNA significantly decreased the expression of GFP. In addition, pre-diluted transfection reagent at room temperature and small nucleic acids increased the transfection efficiency, which peaked at 24 h. Compared with circular nucleic acids, linear nucleic acids showed higher transfection efficiency and a higher genome integration rate. We optimized cationic polymer-mediated RNAi conditions, and our data will be useful for future RNAi studies
Disciplinas: Biología,
Medicina veterinaria y zootecnia
Palabras clave: Genética,
Bovinos,
ARN,
Polimerización,
Cationes,
Transfección
Keyword: Genetics,
Bovines,
RNA,
Transfection,
Polymerization,
Cations
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