Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae)
Document title: Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae)
Journal: Genetics and molecular biology
Database: PERIÓDICA
System number: 000459148
ISSN: 1415-4757
Authors: 1
2
1
Institutions: 1Universidade Federal do Rio Grande do Sul, Departamento de Genetica, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Federal de Ciencias da Saude de Porto Alegre, Departamento de Farmacociencias, Porto Alegre, Rio Grande do Sul. Brasil
Year:
Volumen: 46
Number: 1
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract The association between plants and their pollinators is essential for increasing the diversity in angiosperms. Morphological and physiological traits, mainly floral scent, can influence the pollination dynamics and select pollinators for each plant species. In this work, we studied two proteins involved in producing volatile organic compounds in plants, conyferyl alcohol acyltransferase (CFAT) and benzoyl-CoA:benzyl alcohol/phenyl ethanol benzoyl transferase (BPBT) genes. We aimed to understand these proteins with respect to evolutionary and structural aspects and functions in Solanaceae using phylogenetic methods and comparative molecular modeling. We used Bayesian inference to describe the proteins’ evolutionary history using Petunia x hybrida as a query to search for homologs in the Solanaceae family. Theoretical 3D models were obtained for both proteins using Panicum virgatum as a template. The phylogenetic tree included several different enzymes with diverse biological roles in Solanaceae, displaying the transferase domain. We identified only one sequence of CFAT in the databases, which belongs to Petunia x hybrida, and found several BPBT sequences from the genera Nicotiana, Solanum, and Capsicum. The 3D structures of CFAT and BPBT have two different domains, and we have identified the amino acid residues essential for the enzymatic activity and interaction with substrates
Disciplines: Biología,
Química
Keyword: Angiospermas,
Fitoquímica,
Evolución molecular,
Proteínas,
Compuestos orgánicos volátiles,
Petunia hybrida,
Solanaceae
Keyword: Angiosperms,
Phytochemistry,
Molecular evolution,
Proteins,
Volatile organic compounds,
Petunia hybrida,
Solanaceae
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