| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459154 |
| ISSN: | 1415-4757 |
| Authors: | Ren, Yu1 Wu, Yun2 He, Wenshuai2 Tian, Yingjie2 Zhao, Xingsheng2 |
| Institutions: | 1Inner Mongolia People’s Hospital, Department of Scientific Research, Hohhot. China 2Inner Mongolia People’s Hospital, Department of Cardiology, Hohhot. China |
| Year: | 2023 |
| Volumen: | 46 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Mesenchymal stem cells-derived exosomes (MSCs-exosomes) reportedly possess cardioprotective effects. This study investigated the therapeutic potential and mechanisms of MSCs-exosomes on heart failure (HF). H9c2 cells were used to establish a cardiomyocyte hypertrophy model by angiotensin II (Ang II) treatment. Isolated MSCs-exosomes were identified by transmission electron microscope and CD63 detection. Apoptosis rate was measured by terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. Levels of inflammatory factors [interleukin (IL)-1β, IL-4, IL-6, and tumor necrosis factor (TNF)-α] and brain natriuretic peptide (BNP) were determined by ELISA. Expression of apoptosis-related proteins [Bax, B-cell lymphoma-2 (Bcl-2), and caspase 3] and Hippo-Yes-associated protein (YAP) pathway-related proteins [YAP, phosphor (p)-YAP, and tafazzin (TAZ)] was detected by western blotting. Cardiomyocyte hypertrophy of H9c2 cells induced by Ang II was ameliorated by MSCs-exosomes treatment. MSCs-exosomes downregulated Bax and caspase 3 levels and upregulated Bcl-2 level in Ang II-induced H9c2 cells. MSCs-exosomes also reduced the levels of BNP, IL-1β, IL-4, IL-6, and TNF-α in Ang II-induced H9c2 cells. Meanwhile, p-YAP was downregulated and TAZ was upregulated after MSCs-exosomes administration. In conclusion, MSCs-exosomes alleviate the apoptosis and inflammatory response of cardiomyocyte via deactivating Hippo-YAP pathway in HF |
| Disciplines: | Medicina |
| Keyword: | Sistema cardiovascular, Insuficiencia cardiaca, Exosomas, Células madre mesenquimales, Médula ósea |
| Keyword: | Cardiovascular system, Heart failure, Exosomes, Mesenchymal stem cells, Bone marrow |
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