| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459149 |
| ISSN: | 1415-4757 |
| Authors: | Thompson, Claudia Elizabeth1 Brisolara Corrêa, Lauís2 Thompson, Helen Nathalia3 Stassen, Hubert3 Freitas, Loreta Brandão de2 |
| Institutions: | 1Universidade Federal de Ciencias da Saude de Porto Alegre, Departamento de Farmacociencias, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal do Rio Grande do Sul, Departamento de Genetica, Porto Alegre, Rio Grande do Sul. Brasil 3Universidade Federal do Rio Grande do Sul, Instituto de Quimica, Porto Alegre, Rio Grande do Sul. Brasil |
| Year: | 2023 |
| Volumen: | 46 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Plant RNases T2 are involved in several physiological and developmental processes, including inorganic phosphate starvation, senescence, wounding, defense against pathogens, and the self-incompatibility system. Solanaceae RNases form three main clades, one composed exclusively of S-RNases and two that include S-like RNases. We identified several positively selected amino acids located in highly flexible regions of these molecules, mainly close to the B1 and B2 substrate-binding sites in S-like RNases and the hypervariable regions of S-RNases. These differences between S- and S-like RNases in the flexibility of amino acids in substrate-binding regions are essential to understand the RNA-binding process. For example, in the S-like RNase NT, two positively selected amino acid residues (Tyr156 and Asn134) are located at the most flexible sites on the molecular surface. RNase NT is induced in response to tobacco mosaic virus infection; these sites may thus be regions of interaction with pathogen proteins or viral RNA. Differential selective pressures acting on plant ribonucleases have increased amino acid variability and, consequently, structural differences within and among S-like RNases and S-RNases that seem to be essential for these proteins play different functions |
| Disciplines: | Biología, Química |
| Keyword: | Angiospermas, Fitoquímica, Evolución y filogenia, Solanaceae, Ribonucleasa, S-RNasa |
| Keyword: | Angiosperms, Phytochemistry, Evolution and phylogeny, Solanaceae, Ribonuclease, S-RNase |
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