| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459153 |
| ISSN: | 1415-4757 |
| Authors: | Wairich, Andriele1 Vitali, Ariane2 Adamski, Janete Mariza2 Lopes, Karina Letícia1 Duarte, Guilherme Leitão2 Ponte, Lucas Roani1 Costa, Henrique Keller3 Menguer, Paloma Koprovski1 Santos, Rinaldo Pires dos2 Fett, Janette Palma1 Sperotto, Raul Antonio4 Ricachenevsky, Felipe Klein1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal do Rio Grande do Sul, Instituto de Biociencias, Porto Alegre, Rio Grande do Sul. Brasil 3Universidade Federal de Santa Maria, Instituto de Ciencias Naturais e Exatas, Porto Alegre, Rio Grande do Sul. Brasil 4Universidade do Vale do Taquari, Lajeado, Rio Grande do Sul. Brasil |
| Year: | 2023 |
| Volumen: | 46 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | NAC transcription factors are plant-specific proteins involved in many processes during the plant life cycle and responses to biotic and abiotic stresses. Previous studies have shown that stress-induced OsNAC5 from rice (Oryza sativa L.) is up-regulated by senescence and might be involved in control of iron (Fe) and zinc (Zn) concentrations in rice seeds. Aiming a better understanding of the role of OsNAC5 in rice plants, we investigated a mutant line carrying a T-DNA insertion in the promoter of OsNAC5, which resulted in enhanced expression of the transcription factor. Plants with OsNAC5 enhanced expression were shorter at the seedling stage and had reduced yield at maturity. In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression. Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants. Our work shows that fine-tuning of transcription factors is key when aiming at crop improvement |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Genética, Arroz, Oryza sativa, Factores de transcripción |
| Keyword: | Angiosperms, Genetics, Rice, Oryza sativa, Transcription factors |
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