Journal: | Genetics and molecular biology |
Database: | PERIÓDICA |
System number: | 000346090 |
ISSN: | 1415-4757 |
Authors: | Sastre, Darío A1 Argaraña, Carlos E2 Heller, Viviana B1 Gallo, Mónica3 Fernández, Enrique N1 Rodríguez, Cecilia M1 |
Institutions: | 1Hospital Nacional de Clínicas, Laboratorio de Oncohematología, Córdoba. Argentina 2Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Córdoba. Argentina 3Universidad Católica de Córdoba, Instituto San Agustín, Córdoba. Argentina |
Year: | 2007 |
Season: | Sep |
Volumen: | 30 |
Number: | 3 |
Pages: | 520-523 |
Country: | Brasil |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for e1a2, while in the BCR-ABL(+)AML patients a b3a2 rearrangement was observed. In conclusion, multiplex-PCR allows rapid, specific and simultaneous detection of different types of BCR-ABL transcripts in CML and ABL-BCR(+)AL. A full correlation was observed when multiplex-PCR was compared with nested PCR |
Disciplines: | Medicina |
Keyword: | Diagnóstico, Hematología, Genética, Técnicas de diagnóstico, Diagnóstico genético, PCR, Leucemia mieloide crónica |
Keyword: | Medicine, Diagnosis, Hematology, Genetics, Diagnostic techniques, Genetic diagnosis, PCR, Chronic myeloid leukemia |
Full text: | Texto completo (Ver HTML) |