| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000398048 |
| ISSN: | 0717-3458 |
| Authors: | Kim, Jong-Wan1 Yoe, Jeehyun1 Lee, Gil Ho2 Yoe, Sung Moon1 |
| Institutions: | 1Dankook University, Department of Biological Sciences, Cheonan, Chungnam. Corea del Sur 2Dankook University, College of Medicine, Cheonan, Chungnam. Corea del Sur |
| Year: | 2011 |
| Volumen: | 14 |
| Number: | 3 |
| Pages: | 1-10 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The chicken -type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an antibacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C -terminal fusion protein with glut athione S -transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on- column refolding, and purified by reversed -phase chromatography to 95% purity. The purified rSLLyz demonst rated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for maximizing production and purification of proteins expressed as i nclusion bodies in E. coli |
| Disciplines: | Biología, Química |
| Keyword: | Genética, Insectos, Bioquímica, Actividad antibacteriana, Cuerpos de inclusión, Lisozima, Expresión recombinante, Spodoptera sunia |
| Keyword: | Biology, Chemistry, Genetics, Insects, Biochemistry, Antibacterial activity, Inclusion bodies, Lysozyme, Recombinant expression, Spodoptera litura |
| Full text: | Texto completo (Ver PDF) |
