Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
Document title: Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000358866
ISSN: 0717-3458
Authors: 1
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Institutions: 1Shandong Peanut Research Institute, Qingdao. China
2Qingdao Entry -Exit Inspection and Quarantine Bureau, Qingdao. China
3Jilin Academy of Agricultural Sciences, Institute of Economic Plants, Gongzhuling, Jilin. China
Year:
Volumen: 15
Number: 5
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (109 cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively, were further studied by real time RT-PCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed
Disciplines: Agrociencias,
Biología
Keyword: Plantas para uso industrial,
Bacterias,
Genética,
Cacahuate,
Arachis hypogaea,
Ralstonia solanacearum,
Plantas transgénicas,
Biotecnología
Keyword: Agricultural sciences,
Biology,
Plants for industrial use,
Bacteria,
Genetics,
Peanut,
Arachis hypogaea,
Ralstonia solanacearum,
Transgenic plants,
Biotechnology
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