| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000360055 |
| ISSN: | 0717-3458 |
| Authors: | Westman, Johan O1 Taherzadeh, Mohammad1 Franzen, Carl Johan2 |
| Institutions: | 1University of Boras, School of Engineering, Boras. Suecia 2Chalmers University of Technology, Department of Chemical and Biological Engineering-Industrial Biotechnology, Gotemburgo, Vastra Gotaland. Suecia |
| Year: | 2012 |
| Season: | May |
| Volumen: | 15 |
| Number: | 3 |
| Pages: | 5-5 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to lignocellulose-derived inhibitors, was generally smaller in CCUG53310 than in CBS8066 in inhibitory media. The flocculation of CCUG53310 was linked to the expression of FLO8, FLO10 and one or more of FLO1, FLO5 or FLO9. Flocculation depended on cell wall proteins and Ca2+ ions, but was almost unaffected by other compounds and pH values typical for lignocellulosic media. Conclusions: S. cerevisiae CCUG53310 can be characterised as being very robust, with great potential for industrial fermentation of lignocellulosic hydrolysates relatively low in phenolic inhibitors |
| Disciplines: | Ingeniería, Química, Biología |
| Keyword: | Ingeniería de energéticos, Fermentaciones, Hongos, Biocombustibles, Furfural, Lignocelulosa, Inhibidores fenólicos, Saccharomyces cerevisiae |
| Keyword: | Engineering, Chemistry, Biology, Energy engineering, Fermentation, Fungi, Biofuels, Furfural, Lignocellulose, Phenolic inhibitors, Saccharomyces cerevisiae |
| Full text: | Texto completo (Ver PDF) |
