| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000358226 |
| ISSN: | 0717-3458 |
| Authors: | Tang, Yue Yi1 Wang, Chuan Tang2 Yang, Guan Pin1 Feng, Tong3 Gao, Hua Yuan3 Wang, Xiu Zhen3 Chi, Xiao Yuan2 Xu, Ya Long4 Wu, Qi2 Chen, Dian Xu2 |
| Institutions: | 1Ocean University of China, College of Marine Life Sciences, Qingdao, Shandong. China 2Shandong Peanut Research Institute, Qingdao, Shandong. China 3Jilin Academy of Agricultural Sciences, Institute of Economic Plants, Gongzhuling, Jilin. China 4Zhengzhou Tobacco Research Institute of CNTC, China Tobacco Gene Research Center, Zhengzhou, Henan. China |
| Year: | 2011 |
| Volumen: | 14 |
| Number: | 5 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR) |
| Disciplines: | Biología |
| Keyword: | Fisiología vegetal, Genética, Biotecnología, Tolerancia al frío, Congelación, Respuesta fisiológica, Expresión génica, Cacahuate, Arachis hypogaea |
| Keyword: | Biology, Genetics, Plant physiology, Biotechnology, Cold tolerance, Chilling, Physiological response, Gene expression, Peanut, Arachis hypogaea |
| Full text: | Texto completo (Ver HTML) |
