| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000359297 |
| ISSN: | 0717-3458 |
| Authors: | Osorio, Mayra1 Gámez, Elizabeth1 Molina, Sandy1 Infante, Diógenes1 |
| Institutions: | 1Centro Nacional de Biotecnología Agrícola, Instituto de Estudios Avanzados, Caracas, Distrito Federal. Venezuela |
| Year: | 2012 |
| Volumen: | 15 |
| Number: | 4 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the beginning of the somatic embryogenesis process were grouped as independent of the other plants when analyzed at the acclimatization stage. The differences found with the different markers used are discussed. In field trials, micropropagated plants had a yield between 4 and 5 times the average yield of cassava in Venezuela. Conclusion: Despite variability in terms of DNA markers, somatic embryogenesis is suitable for mass propagation of highly performing cassava clones |
| Disciplines: | Biología, Química |
| Keyword: | Biotecnología, Embriogénesis somática, Micropropagación, Estadios de desarrollo, Estabilidad genética, Marcadores moleculares, Manihot esculenta |
| Keyword: | Biology, Chemistry, Biotechnology, Somatic embryogenesis, Micropropagation, Developmental stages, Genetic stability, Molecular markers, Manihot esculenta |
| Full text: | Texto completo (Ver HTML) |
