| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000360047 |
| ISSN: | 0717-3458 |
| Autores: | Ahmed, Sibtain2 Imdad, Syeda Sana1 Jamil, Amer1 |
| Instituciones: | 1University of Agriculture, Department of Chemistry and Biochemistry, Faisalabad. Pakistán 2University of New Mexico, School of Medicine, Albuquerque, Nuevo México. Estados Unidos de América |
| Año: | 2012 |
| Periodo: | May |
| Volumen: | 15 |
| Número: | 3 |
| Paginación: | 3-3 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T. harzianum using oat spelt xylan as a substrate were 4.8 mg mL-1 and 0.526 μmol min-1 mg-1, respectively. Whereas values of the partially purified xylanase from C. thermophilum were 2.96 mg mL-1 and 0.25 μmol min-1 mg-1, respectively. These findings in this study have great implications for the future applications of xylanases |
| Disciplinas: | Biología, Química |
| Palabras clave: | Hongos, Bioquímica, Fermentaciones, Chaetomium thermophilum, Cinética, Trichoderma harzianum, Xilanasa |
| Keyword: | Biology, Chemistry, Fungi, Biochemistry, Fermentation, Chaetomium thermophilum, Kinetics, Trichoderma harzianum, Xylanases |
| Texto completo: | Texto completo (Ver PDF) |
