Journal: | Electronic journal of biotechnology |
Database: | PERIÓDICA |
System number: | 000266099 |
ISSN: | 0717-3458 |
Authors: | Ikram-ul-Haq1 Ali, Sikander Qadeer, M.A Iqbal, Javed2 |
Institutions: | 1Government College, Department of Botany, Lahore, Punjab. Pakistán 2University of the Punjab, Department of Botany, Lahore, Punjab. Pakistán |
Year: | 2002 |
Season: | Ago |
Volumen: | 5 |
Number: | 2 |
Country: | Chile |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | Citric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated molasses [K4Fe(CN)6 200 ppm] medium containing sugar 150 g/l was employed as the basal fermentation medium. Different cultural conditions such as incubation temperature (30ºC), initial pH (6.0), air supply (1.0 l-1l-1min), agitation intensity (200 rpm) and time profile (144 h after inoculation) were optimised for enhanced citric acid production. Maximum amount of anhydrous citric acid obtained during the course of study was 106.65 g/l, with a sugar consumption of 107 g/l. Final pH, ferrocyanide concentration and dry cell mass were 2.1, 60 ppm and 16.5 g/l, respectively |
Disciplines: | Química, Biología |
Keyword: | Fermentaciones, Hongos, Biotecnología, Aspergillus niger, Mutantes, Acido cítrico, Melaza, Caña de azúcar |
Keyword: | Chemistry, Biology, Fermentation, Fungi, Aspergillus niger, Mutants, Citric acid, Sugar cane, Molasses |
Full text: | Texto completo (Ver HTML) |