| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000359302 |
| ISSN: | 0717-3458 |
| Authors: | Ramírez, Ingrid1 Dorta, Fernando1 Cuadros Inostroza, Alvaro2 Peña Cortés, Hugo1 |
| Institutions: | 1Universidad Técnica Federico Santa María, Centro de Biotecnología D. Alkalay L., Valparaíso. Chile 2Max Planck Society, Institute of Molecular Plant Physiology, Potsdam, Brandenburg. Alemania |
| Year: | 2012 |
| Volumen: | 15 |
| Number: | 4 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite |
| Disciplines: | Biología |
| Keyword: | Fisiología vegetal, Regeneración vegetal, Inducción de callos, Cultivo de tejidos, Antioxidantes, Ulex europaeus, Fabaceae |
| Keyword: | Biology, Plant physiology, Plant regeneration, Callus induction, Tissue culture, Antioxidants, Ulex europaeus, Fabaceae |
| Full text: | Texto completo (Ver HTML) |
