| Journal: | Computación y sistemas |
| Database: | |
| System number: | 000560760 |
| ISSN: | 1405-5546 |
| Authors: | Portuondo Mallet, Lariza M.1 Chinea Valdés, Lyanett1 Orozco Morales, Rubén3 Lorenzo Ginori, Juan V.1 |
| Institutions: | 1Universidad Central Marta Abreu de las Villas, Centro de Investigaciones de la Informática, Villa Clara. Cuba 2Universidad de Oriente, Centro de Estudios de Neurociencias Procesamiento de Imágenes y Señales (CENPIS), México 3Universidad Central Marta Abreu de las Villas, Centro de Estudio de Métodos Computacionales y Numéricos en la Ingeniería (CEMNI), Villa Clara. Cuba |
| Year: | 2022 |
| Season: | Oct-Dic |
| Volumen: | 26 |
| Number: | 4 |
| Pages: | 1569-1586 |
| Country: | México |
| Language: | Inglés |
| English abstract | Segmentation of clusters of erythrocytes into their constituent single cells is a procedure needed in various biomedical applications related to microscopy images. This task is part of the general problem of splitting clumps of objects in images which continues being an open research topic in the Image Processing field. This work presents a unified morphological method to detect and segment clusters of erythrocytes in microscopy images, and proposes two main contributions. The first one is to formulate and evaluate a method to detect clusters as connected components in binary images, obtained from a previous coarse segmentation, which is not capable of further dividing a cluster into its constituent cells. Secondly, to propose the best alternative to split the clusters into their constituent individual cells after evaluating three algorithms based in the combination of the transforms: H-maxima, weighted external distance and marker-controlled watershed. Evaluation of the proposed cluster detection methods was made in terms of standard measures of effectiveness. Segmentation accuracy was evaluated comparing the segmented objects obtained to a manually segmented ground truth, by means of the Jaccard index. Then the Friedman test allowed validating the advantages of the proposed method in comparison to the other alternatives studied here. |
| Keyword: | Image segmentation, Clusters splitting, Watersheds, Distance transform |
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