Journal: | Brazilian Journal of Pharmaceutical Sciences |
Database: | PERIÓDICA |
System number: | 000451343 |
ISSN: | 1984-8250 |
Authors: | Dong, Wenzhu1 Chen, Hangping1 Wang, Lu1 Cao, Xiaoqian1 Bu, Xiawei1 Peng, Yan1 Dong, Aiqing2 Ying, Mengjiang2 Chen, Xu2 Zhang, Xin2 Yao, Li1 |
Institutions: | 1Zhejiang Chinese Medical University, College of Pharmaceutical Science, Hangzhou, Zhejiang. China 2Taizhou Central Hospital, Taizhou, Zhejiang. China |
Year: | 2020 |
Volumen: | 56 |
Country: | Brasil |
Language: | Inglés |
Document type: | Artículo |
Approach: | Analítico, descriptivo |
English abstract | Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed to classify the genes. Visualization and Integrated Discovery (DAVID) database and Gene Ontology database were used to explore the biological function. Finally, Onto-tools Pathway Express was used to analyze the pathways of shared genes. Importantly, upregulated common genes, such as Bad, Orm1, Arntl and Zbtb7a, were used to construct a network of 150 genes, while downregulated genes, such as Mif and Gpx1, formed a network of 29 genes. Interestingly, the networks were involved in various pathways, such as insulin signal pathway, endometrial cancer pathway, circadian rhythm pathway, and pancreatic cancer pathway. We discovered common genes of SHR, GK and HMR compared with normal Wistar rats, and the association of these genes together with biological function were preliminarily revealed |
Disciplines: | Medicina |
Keyword: | Genética, Patología, Hipertensión, Diabetes, Hiperlipidemia, Fisiopatogenia, Control genético, Microarreglos genéticos |
Keyword: | Genetics, Pathology, Hypertension, Diabetes, Hyperlipidemia, Physiopathogenesis, Genetic control, Genetic microarrays |
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