Exploring the shared genes of hypertension, diabetes and hyperlipidemia based on microarray



Título del documento: Exploring the shared genes of hypertension, diabetes and hyperlipidemia based on microarray
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451343
ISSN: 1984-8250
Autors: 1
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Institucions: 1Zhejiang Chinese Medical University, College of Pharmaceutical Science, Hangzhou, Zhejiang. China
2Taizhou Central Hospital, Taizhou, Zhejiang. China
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Volum: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed to classify the genes. Visualization and Integrated Discovery (DAVID) database and Gene Ontology database were used to explore the biological function. Finally, Onto-tools Pathway Express was used to analyze the pathways of shared genes. Importantly, upregulated common genes, such as Bad, Orm1, Arntl and Zbtb7a, were used to construct a network of 150 genes, while downregulated genes, such as Mif and Gpx1, formed a network of 29 genes. Interestingly, the networks were involved in various pathways, such as insulin signal pathway, endometrial cancer pathway, circadian rhythm pathway, and pancreatic cancer pathway. We discovered common genes of SHR, GK and HMR compared with normal Wistar rats, and the association of these genes together with biological function were preliminarily revealed
Disciplines Medicina
Paraules clau: Genética,
Patología,
Hipertensión,
Diabetes,
Hiperlipidemia,
Fisiopatogenia,
Control genético,
Microarreglos genéticos
Keyword: Genetics,
Pathology,
Hypertension,
Diabetes,
Hyperlipidemia,
Physiopathogenesis,
Genetic control,
Genetic microarrays
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