| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451959 |
| ISSN: | 1984-8250 |
| Authors: | Dutra, Flávia Viana Avelar1 Teixeira, Leila Suleimara1 Pires, Bruna Carneiro1 Florez, Diego Hernando Ângulo1 Teixeira, Roseane Andrade1 Borges, Keyller Bastos1 |
| Institutions: | 1Universidade Federal de Sao Joao del Rei, Departamento de Ciencias Naturais, Sao Joao del Rei, Minas Gerais. Brasil |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile: ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the field |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Química analítica, Avermectina, Milbemicina, Muestras de granel, Control de calidad, Cromatografía líquida de alta resolución (HPLC) |
| Keyword: | Medicinal chemistry, Analytical chemistry, High performance liquid chromatography (HPLC), Avermectin, Milbemycin, Quality control, Bulk samples |
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