Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples



Título del documento: Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451959
ISSN: 1984-8250
Autores: 1
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Instituciones: 1Universidade Federal de Sao Joao del Rei, Departamento de Ciencias Naturais, Sao Joao del Rei, Minas Gerais. Brasil
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile: ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the field
Disciplinas: Química
Palabras clave: Química farmacéutica,
Química analítica,
Avermectina,
Milbemicina,
Muestras de granel,
Control de calidad,
Cromatografía líquida de alta resolución (HPLC)
Keyword: Medicinal chemistry,
Analytical chemistry,
High performance liquid chromatography (HPLC),
Avermectin,
Milbemycin,
Quality control,
Bulk samples
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