| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000452169 |
| ISSN: | 1984-8250 |
| Authors: | Moatamedi, Nasim1 Emamzadeh, Rahman2 Sadeghi, Hamid Mir Mohammad1 Akbari, Vajihe1 |
| Institutions: | 1Isfahan University of Medical Sciences, Faculty of Pharmacy, Isfahán. Irán 2University of Isfahan, Faculty of Sciences, Isfahán. Irán |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Interferon-β-1a (INF-β-1a) has gained significant attention due to its emerging applications in the treatment of different human diseases. Therefore, many researchers have attempted to produce it in large quantities and also in a biologically active form using different expression systems. In the present study, we aimed to improve the expression level of INF-β-1a by Pichia pastoris using optimization of culture conditions. The codon-optimized INF-β- 1a gene was cloned into pPICZαA plasmid under the control of alcohol oxidase I (AOX1) promoter. The protein expression was induced using different concentrations of methanol at different pHs and temperatures. The biological activity of produced protein was evaluated by anti-proliferative assay. The ideal culture conditions for the expression of INF-β-1a by P. pastoris were found to be induction with 2% methanol at pH 7.0 culture medium at 30 C which yielded a concentration of 15.5 mg/L INF-β-1a in a shake flask. Our results indicate that differences in glycosylation pattern could result in different biological activities as INF- β-1a produced by P. pastoris could significantly more reduce the cell viability of HepG-2 cells, a hepatocellular carcinoma cell line, than a commercially available form of this protein produced by CHO |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Biotecnología, Interferón beta 1-A, Bioprocesos, Expresión génica, Carcinoma hepatocelular, Apoptosis, Pichia pastoris |
| Keyword: | Medicinal chemistry, Biotechnology, Interferon beta 1-A, Bioprocesses, Gene expression, Hepatocellular carcinoma, Apoptosis, Pichia pastoris |
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