Biochemical and histopathological evaluations of chronic renal failure rats treated with pluripotent human stem cells
Document title: Biochemical and histopathological evaluations of chronic renal failure rats treated with pluripotent human stem cells
Journal: Brazilian Journal of Pharmaceutical Sciences
Database: PERIÓDICA
System number: 000451770
ISSN: 1984-8250
Authors: 1
2
1
1
Institutions: 1Damietta University, Faculty of Science, Damietta. Egipto
2University of Tabuk, Faculty of Science, Tabuk. Arabia Saudita
Year:
Volumen: 58
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Regeneration of damaged kidney cells using stem cells is the current research approach in the treatment of chronic renal failure (CRF). In the present study, the histopathological and biochemical techniques were used to evaluate stem cells’ (SCs) role in treatment of CRF. Sixty-four rats were divided into eight groups. Group I (GI): rats were injected with doxorubicin (15 mg/kg) to initiate CRF. GII-GVII: rats were injected with doxorubicin and treated with SCs (1x106 MSCs or/and 2x104 HSCs/rat) with/without growth factors extract (200 µL/rat) and/or immunosuppressor (cyclosporine A, 5 mg/kg/day). GVIII: rats treated with PBS (100 µL/kg/day). Levels of creatinine, urea and uric acid were increased in rats sera after injection with doxorubicin, while blood electrolyte levels of Na, K, P and Mg were decreased. Also, histopathological abnormalities such as hyalinized blood vessels, degenerated hyalinized glomerulus tubules and cell debris in the lumen and degeneration of renal tissues were observed in these rats. After treatment with SCs, all these parameters restore their normal values with regeneration of the damaged cells as demonstrated in histopathology of the treated groups. It can be concluded that, the use of SCs in treatment of kidney diseases is a promising approach and needs more efforts
Disciplines: Medicina
Keyword: Nefrología,
Medicina experimental,
Insuficiencia renal crónica (IRC),
Regeneración celular,
Células troncales hemotopoyéticas,
Células troncales mesenquimatosas,
Factor de crecimiento,
Electrólitos
Keyword: Nephrology,
Experimental medicine,
Chronic renal failure (CRF),
Cell regeneration,
Hematopoietic stem cells,
Mesenchymal stem cells,
Growth factor,
Electrolytes
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