| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000452136 |
| ISSN: | 1984-8250 |
| Authors: | Tian, Zhen1 Li, Zhitao2 Guo, Tian2 Li, He2 Mu, Yanshuang1 |
| Institutions: | 1Northeast Agricultural University, Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Harbin, Heilongjiang. China 2Harbin Medical University, Key Laboratory of Cardiovascular Pathophysiology, Harbin, Heilongjiang. China |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The present study was designed to examine the effects of atorvastatin on vascular inflammatory responses in human coronary artery endothelial cells(HCAECs), when challenged by lipopolysaccharide (LPS), a Toll-like receptor-4 (TLR4) ligand. HCAECs were pretreated with atorvastatin and induced by LPS. The expression of TLR4, interleukin -6(IL-6), monocyte chemoattractant protein 1(MCP-1), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecular-1(ICAM-1), nuclear factor-κB (NF-κB) and p38 mitogen activated protein kinase(p38 MAPK) were evaluated using Real-time polymerase chain reaction, cytokine ELISA assay and Western blotting. The results showed that pretreatment with atorvastatin down-regulated the expression of TLR4 in LPS-activated HCAECs. Atorvastatin also attenuated the LPS-induced expression of interleukin IL-6 and MCP-1, at both the transcription and translation level in HCAECs. LPS-induced endothelial cell adhesion molecules, ICAM-1 and VCAM-1 expression were also reduced by pretreatment with atorvastatin. Furthermore, atorvastatin efficiently suppressed LPS-induced phosphorylation of NF-κB and p38 MAPK in HCAECs. These findings show that atorvastatin suppresses endothelial cell inflammation, suggesting that atorvastatin may be suitable for development as a therapeutic agent for inflammatory cardiovascular disease |
| Disciplines: | Medicina |
| Keyword: | Sistema cardiovascular, Farmacología, Inflamación arterial, Lipopolisacáridos, Células endoteliales, Atorvastatina |
| Keyword: | Cardiovascular system, Pharmacology, Arterial inflammation, Atorvastatin, Lipopolysaccharides, Endothelial cells |
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