| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000452175 |
| ISSN: | 1984-8250 |
| Authors: | Meneses, María Laura1 Recalde, Maite2 Martin, Paula Lorena3 Pardo, Alejandro Guillermo1 |
| Institutions: | 1Universidad Nacional de Quilmes, Instituto de Microbiología Básica y Aplicada, Quilmes, Buenos Aires. Argentina 2Universidad Nacional Arturo Jauretche, Instituto de Ingeniería y Agronomía, Buenos Aires. Argentina 3Universidad Nacional de La Plata, Hospital Escuela, Buenos Aires. Argentina |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The aim of present study was calculate the Minimum inhibitory concentrations (MICs) of silver nanoparticles and clotrimazole for Candida species and their interaction by the adaptation of standarized methods. The MICs values of clotrimazole were 9 E-04-3 E-03 ug/ml, 0.1-0.6 ug/ml, 3 E-03- 0.1 ug/ml and 3 E-03-0.3 ug/ml for Candida albicans susceptible to fluconazole, Candida albicans resistance to fluconazole, Candida krusei and Candida parapsilosis respectively. The MICs values of silver nanoparticles were 26.50- 53 ug/ml; 26.50-106 ug/ml; 106-212 ug/ ml and 26.50- 53 ug/ml for Candida albicans susceptible to fluconazole, Candida albicans resistance to fluconazole, Candida krusei and Candida parapsilosis respectively. Synergism between clotrimazole and silver nanoparticles was measured by checkerboard BMD (broth microdilution) test and shown only for C. albicans susceptible to fluconazole because the fractional inhibitory concentrations (FICs) values were 0.07 - 0.15 ug/ml. Indifference was shown for the other species tested because the FICs values were between 0.5 - 2- 3.06 ug/ml. The results suggest synergistic activity depending on the fungus species analysed, however we recommend the incorporation of others measurement methodologies to confirm our results. As for measurement methodologies of MICs of silver nanoparticles and clotrimazole international normative were respected to guarantee reproducible and comparable results |
| Disciplines: | Química |
| Keyword: | Farmacología, Microbiología, Clotrimazol, Nanopartículas de plata, Actividad antifúngica, Candida |
| Keyword: | Pharmacology, Microbiology, Clotrimazole, Silver nanoparticles, Antifungal activity, Candida |
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