Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in chicken carcasses and pork meat



Document title: Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in chicken carcasses and pork meat
Journal: Brazilian journal of microbiology
Database: PERIÓDICA
System number: 000291237
ISSN: 1517-8382
Authors: 1
2

3



Institutions: 1Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias, Florianopolis, Santa Catarina. Brasil
2Universidade Federal de Santa Catarina, Department of Informatics and Statistics, Florianopolis, Santa Catarina. Brasil
3Centro de Tecnologia da Perdigao S/A, Laboratorio de Microbiologia, Sao Paulo. Brasil
Year:
Season: Oct-Dic
Volumen: 37
Number: 4
Pages: 521-526
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental
English abstract A study was performed to compare the analytical procedure of the BAX® System for Salmonella PCR assay with the Modified Semi-Solid Rappaport-Vassiliadis (MSRV) method, for the detection of Salmonella in naturally contaminated chicken carcass samples (n = 762) and raw pork meat (n = 566). The chicken carcasses samples were collected during slaughtering after defeathering or immediately after evisceration and the raw pork meat collected from the deboned head of recently slaughtered pigs and others deboned raw fresh pork meat. The BAX® System detected 134 Salmonella-positive samples in chicken carcasses and 145 samples in pork meat, while the MSRV method isolated 142 and 144 Salmonella-positive samples, respectively. No significant difference was observed between the two methods for chicken carcasses and pork meat, according to McNemar test at the 5% level
Portuguese abstract Um estudo foi realizado com o objetivo de comparar o procedimento analítico de detecção de Salmonella com o Sistema BAX® automatizado, baseado na Reação em Cadeia da Polimerase (PCR) com o método de Rappaport-Vassiliadis em Agar Semi-Sólido modificado (MSRV) para detecção de Salmonella em amostras de carcaças de frango naturalmente contaminadas (n=762) e retalhos de carne suía (n=566). O Sistema BAX® detectou 134 amostras positivas para Salmonella em carcaças de frango e 145 amostras positivas para Salmonella em retalhos de carne suína, enquanto o MSRV detectou 142 e 144 amostras positivas respectivamente. Não houve diferença estatisticamente significativa entre os dois métodos, segundo McNemar ao nível de significância de 5%
Disciplines: Química,
Biología
Keyword: Bioquímica,
Química de alimentos,
Bacterias,
Sistema BAX®,
Método MSRV,
Salmonella,
Pollos,
Carne de cerdo
Keyword: Chemistry,
Biology,
Biochemistry,
Food chemistry,
Bacteria,
BAX® System,
MSRV method,
Salmonella,
Chicken,
Pork meat
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