Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus



Document title: Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus
Journal: Brazilian journal of medical and biological research
Database: PERIÓDICA
System number: 000350323
ISSN: 0100-879X
Authors: 1
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1
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Institutions: 1Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo. Brasil
Year:
Season: May
Volumen: 40
Number: 5
Pages: 633-637
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 103/µL; P < 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P < 0.001 and 4.4 ± 0.3 vs 0.9 ± 0.1 103/µL; P < 0.00001, respectively). Both 24- and 96-h SD induced a reduction in the number of lymphocytes in female Swiss (7.5 ± 0.5 vs 4.5 ± 0.5, 4.4 ± 0.6 103/µL; P < 0.001, respectively) and NOD mice (4 ± 0.6 vs 1.8 ± 0.2, 1.2 ± 0.4 103/µL; P < 0.01, respectively) compared to the respective controls. Loss of sleep induced lymphopenia in peripheral blood in both genders and strains used. Since many cases of autoimmunity present reduced numbers of lymphocytes and, in this study, it was more evident in the NOD strain, our results suggest that SD should be considered a risk factor in the onset of autoimmune disorders
Disciplines: Medicina
Keyword: Medicina experimental,
Inmunología,
Diabetes,
Linfopenia,
Privación de sueño,
Estrés
Keyword: Medicine,
Experimental medicine,
Immunology,
Diabetes,
Sleep deprivation,
Lymphopenia,
Stress
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