| Journal: | Brazilian journal of medical and biological research |
| Database: | PERIÓDICA |
| System number: | 000351235 |
| ISSN: | 0100-879X |
| Authors: | Barbosa, A.P.F1 Silva, J.D.P1 Fonseca, E.C2 Lopez, P.M3 Fernandes, M.B.C3 Balduino, A4 Duarte, M.E.L3 |
| Institutions: | 1Universidade Estadual de Ciencias da Saude de Alagoas, Departamento de Patologia, Maceio, Alagoas. Brasil 2Universidade Federal Fluminense, Departamento de Patologia, Niteroi, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Departamento de Histologia e Embriologia, Rio de Janeiro. Brasil 4Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro. Brasil |
| Year: | 2007 |
| Season: | Ago |
| Volumen: | 40 |
| Number: | 8 |
| Pages: | 1101-1109 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Children with chronic renal failure in general present growth retardation that is aggravated by corticosteroids. We describe here the effects of methylprednisolone (MP) and recombinant human growth hormone (rhGH) on the growth plate (GP) of uremic rats. Uremia was induced by subtotal nephrectomy in 30-day-old rats, followed by 20 IU kg-1 day-1 rhGH (N = 7) or 3 mg kg-1 day-1 MP (N = 7) or 20 IU kg-1 day-1 rhGH + 3 mg kg-1 day-1 MP (N = 7) treatment for 10 days. Control rats with intact renal function were sham-operated and treated with 3 mg kg-1 day-1 MP (N = 7) or vehicle (N = 7). Uremic rats (N = 7) were used as untreated control animals. Structural alterations in the GP and the expression of anti-proliferating cell nuclear antigen (PCNA) and anti-insulin-like growth factor I (IGF-I) by epiphyseal chondrocytes were evaluated. Uremic MP rats displayed a reduction in the proliferative zone height (59.08 ± 4.54 vs 68.07 ± 7.5 µm, P < 0.05) and modifications in the microarchitecture of the GP. MP and uremia had an additive inhibitory effect on the proliferative activity of GP chondrocytes, lowering the expression of PCNA (19.48 ± 11.13 vs 68.64 ± 7.9% in control, P < 0.0005) and IGF-I (58.53 ± 0.96 vs 84.78 ± 2.93% in control, P < 0.0001), that was counteracted by rhGH. These findings suggest that in uremic rats rhGH therapy improves longitudinal growth by increasing IGF-I synthesis in the GP and by stimulating chondrocyte proliferation |
| Disciplines: | Medicina |
| Keyword: | Endocrinología, Medicina experimental, Corticosteroides, Hormona del crecimiento, Falla renal, Retraso del crecimiento |
| Keyword: | Medicine, Endocrinology, Experimental medicine, Corticosteroids, Growth hormone, Renal failure, Growth retardation |
| Full text: | Texto completo (Ver HTML) |
