| Journal: | Brazilian journal of medical and biological research |
| Database: | PERIÓDICA |
| System number: | 000351250 |
| ISSN: | 0100-879X |
| Authors: | Carvalho-Junior, A.D1 Vieira, F.P1 Melo, V.J. de2 Lopes, M.T.P2 Silveira, J.N1 Ramaldes, G.A1 Garnier-Suillerot, A4 Pereira-Maia, E.C3 Oliveira, M.C. de1 |
| Institutions: | 1Universidade Federal de Minas Gerais, Faculdade de Farmacia, Belo Horizonte, Minas Gerais. Brasil 2Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal de Minas Gerais, Instituto de Ciencias Exatas, Belo Horizonte, Minas Gerais. Brasil 4Universite de Paris XIII (Paris-Nord), Laboratoire BioMoCeTi, París. Francia |
| Year: | 2007 |
| Season: | Ago |
| Volumen: | 40 |
| Number: | 8 |
| Pages: | 1149-1157 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl hemisuccinate (CHEMS), and distearoylphosphatidylethanolamine-polyethyleneglycol 2000 (DSPE-PEG2000) and were characterized by determining the encapsulation percentage as a function of lipid concentration. Among the different formulations, DOPE/CHEMS/DSPE-PEG liposomes (lipid concentration equal to 40 mM) encapsulated cisplatin more efficiently than other concentrations of liposomes (about 20.0%, mean diameter of 174 nm). These liposomes presented good stability in mouse plasma which was obtained using a 0.24-M EDTA solution (70% cisplatin was retained inside the liposomes after 30 min of incubation). Concerning cytotoxic effects, they are more effective (1.34-fold) than free cisplatin for growth inhibition of the human lung cancer cell line A549. The study of cytotoxicity to GLC4 and GLC4/CDDP cell lines showed similar IC50 values (approximately 1.4 µM), i.e., cisplatin-resistant cells were sensitive to this cisplatin formulation. Platinum accumulation in both sensitive and resistant cell lines followed the same pattern, i.e., approximately the same intracellular platinum concentration (4.0 x 10-17 mol/cell) yielded the same cytotoxic effect. These results indicate that long-circulating pH-sensitive liposomes, also termed as stealth pH-sensitive liposomes, may present a promising delivery system for cisplatin-based cancer treatment. This liposome system proved to be able to circumvent the cisplatin resistance, whereas it was not observed when using non- |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Neumología, Oncología, Cáncer, Pulmones, Cisplatino, Citotoxicidad, Liposomas, Encapsulación |
| Keyword: | Medicine, Oncology, Pharmacology, Pneumology, Lungs, Cancer, Cisplatin, Cytotoxicity, Liposomes, Encapsulation |
| Full text: | Texto completo (Ver HTML) |
