| Journal: | Brazilian journal of medical and biological research |
| Database: | PERIÓDICA |
| System number: | 000350940 |
| ISSN: | 0100-879X |
| Authors: | Dentillo, D.B1 Souza, F.R.P1 Meola, J1 Vieira, G.S1 Yazlle, M.E.H.D1 Goulart, L.R2 Martelli, L1 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 2Universidade Federal de Uberlandia, Instituto de Genetica e Bioquimica, Uberlandia, Minas Gerais. Brasil |
| Year: | 2007 |
| Season: | Jun |
| Volumen: | 40 |
| Number: | 6 |
| Pages: | 793-797 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Pregnancy loss can be caused by several factors involved in human reproduction. Although up to 50% of cases remain unexplained, it has been postulated that the major cause of failed pregnancy is an error of embryo implantation. Transmembrane mucin-1 (MUC-1) is a glycoprotein expressed on the endometrial cell surface which acts as a barrier to implantation. The gene that codes for this molecule is composed of a polymorphic tandem repeat of 60 nucleotides. Our objective was to determine if MUC-1 genetic polymorphism is associated with implantation failure in patients with a history of recurrent abortion. The study was conducted on 10 women aged 25 to 35 years with no history of successful pregnancy and with a diagnosis of infertility. The control group consisted of 32 patients aged 25 to 35 years who had delivered at least two full-term live children and who had no history of abortions or fetal losses. MUC-1 amplicons were obtained by PCR and observed on agarose and polyacrylamide gel after electrophoresis. Statistical analysis showed no significant difference in the number of MUC-1 variable number of tandem repeats between these groups (P > 0.05). Our results suggest that there is no effect of the polymorphic MUC-1 sequence on the implantation failure. However, the data do not exclude MUC-1 relevance during embryo implantation. The process is related to several associated factors such as the mechanisms of gene expression in the uterus, specific MUC-1 post-translational modifications and appropriate interactions with other molecules during embryo implantation |
| Disciplines: | Medicina |
| Keyword: | Ginecología y obstetricia, Infertilidad femenina, Implantación de embriones, Mucina, Polimorfismo genético, Reproducción humana |
| Keyword: | Medicine, Gynecology and obstetrics, Female infertility, Embryo implant, Mucin, Genetic polymorphism, Human reproduction |
| Full text: | Texto completo (Ver HTML) |
