| Revista: | Brazilian journal of medical and biological research |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000351243 |
| ISSN: | 0100-879X |
| Autores: | Barros, L.F.M1 Barros, P.S.M1 Ropke, C.D2 Silva, V.V2 Sawada, T.C.H2 Barros, S.B.M2 Belfort-Junior, R3 |
| Instituciones: | 1Universidade de Sao Paulo, Faculdade de Medicina Veterinaria e Zootecnia, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil 3Universidade Federal de Sao Paulo, Instituto de Visao, Sao Paulo. Brasil |
| Año: | 2007 |
| Periodo: | Ago |
| Volumen: | 40 |
| Número: | 8 |
| Paginación: | 1129-1132 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs was increased compared with untreated eyes. When the gels were incubated with P. umbellata extract the activity of MMP-2, pro-MMP-2 and MMP-9 decreased in a dose-dependent manner. MMP-9 activity decreased by approximately 50% after incubation with 50 µg/mL and was completely abolished at 100 and 250 µg/mL of the extract. After incubation with 50 µg/mL the activity of pro-MMP-2 and MMP-2 also decreased by 50%. The activity of pro-MMP-2 was almost completely abolished after incubation with 250 µg/mL of the extract. For MMP-2 the incubation with 100 or 250 µg/mL of the extract of P. umbellata promoted a 10-fold decrease in activity. In conclusion, P. umbellata root crude extract can be useful as an alternative therapy to control MMP activity after corneal injury |
| Disciplinas: | Medicina |
| Palabras clave: | Medicina experimental, Oftalmología, Traumatología, Córnea, Metaloproteinasas, Extractos vegetales, Pothomorphe umbellata, Quemaduras químicas, Piperaceae |
| Keyword: | Medicine, Experimental medicine, Ophthalmology, Traumatology, Cornea, Chemical burns, Metalloproteinases, Plant extracts, Pothomorphe umbellata, Piperaceae |
| Texto completo: | Texto completo (Ver HTML) |
