| Journal: | Brazilian journal of medical and biological research |
| Database: | PERIÓDICA |
| System number: | 000350928 |
| ISSN: | 0100-879X |
| Authors: | Zeng, Y1 Song, C1 Ding, X1 Ji, X2 Yi, L3 Zhu, K3 |
| Institutions: | 1Nanjing Medical University, The First Affiliated Hospital, Nanjing, Jiangsu. China 2Nanjing Medical University, The Second Affiliated Hospital, Nanjing, Jiangsu. Brasil 3General Hospital of PLA, Department of Neurology, Beijing. China |
| Year: | 2007 |
| Season: | Jul |
| Volumen: | 40 |
| Number: | 7 |
| Pages: | 1003-1010 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Scutellaria baicalensis Georgi is one of the important medicinal herbs widely used for the treatment of various inflammatory diseases in Asia. Baicalin (BA) is a bioactive anti-inflammatory flavone found abundantly in Scutellaria baicalensis Georgi. To explore the therapeutic potential of BA, we examined the effects of systemic administration of the flavone (5 and 10 mg/kg, ip) on relapsing/remitting experimental autoimmune encephalomyelitis (EAE) induced by proteolipid protein 139-151 in SJL/J mice, an experimental model of multiple sclerosis. The mice treated with PBS or BA at day -1 and for 3 consecutive days were observed daily for clinical signs of disease up to 60 days after immunization. In the PBS-EAE group, neurological scores were: incidence (100%), mean day of onset (8.0 ± 0.73), peak clinical score (3.0 ± 0.4), and cumulative disease index (141.8 ± 19.4). In the BA-EAE group (5 or 10 mg kg-1 day-1, respectively), incidence (95 or 90%), mean day of onset (9.0 ± 0.80 or 9.2 ± 0.75; P = 0.000), peak clinical score (2.2 ± 0.3 or 2.0 ± 0.3; P = 0.000), and cumulative disease index (75.9 ± 10.1 or 62.9 ± 8.4; P = 0.000) decreased, accompanied by the histopathological findings (decrease of dense mononuclear infiltration surrounding vascellum) for the spinal cord. Additionally, the in vitro effects of BA (5, 10, and 25 µM) on mononuclear cells collected from popliteal and inguinal lymph nodes of day-10 EAE mice were evaluated using an MTT reduction assay for cell proliferation, and ELISA to measure IFN-g and IL-4 cytokines. Compared with the control group, BA caused an increase in IL-4 (EAE-DMSO: 3.56 ± 0.42 pg/mL vs EAE-BA (5, 10, and 25 µM): 6.03 ± 1.1, 7.83 ± 0.65, 10.54 ± 1.13 pg/mL, respectively; P < 0.001); but inhibited IFN-g (EAE-DMSO: 485.76 ± 25.13 pg/mL vs EAE-BA (5, 10, and 25 µM): 87.08 ± 9.24, 36.27 ± 5.44, 19.18 ± 2.93 pg/mL, respectively; P < 0.001) and the proliferation of mononuclear cells (EAE-DMSO: 0.73 ± 0.021 vs |
| Disciplines: | Medicina |
| Keyword: | Medicina experimental, Neurología, Plantas medicinales, Encefalomielitis autoinmune, Flavonas, Baicalina, Esclerosis múltiple, Scutellaria baicalensis |
| Keyword: | Medicine, Experimental medicine, Neurology, Medicinal plants, Autoimmune encephalomyelitis, Flavones, Baicalin, Multiple sclerosis, Scutellaria baicalensis |
| Full text: | Texto completo (Ver HTML) |
