| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308919 |
| ISSN: | 0104-6632 |
| Authors: | Rangel-Yagui, C. O1 Pessoa-Junior, A Blankschtein, D2 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil 2Massachusetts Institute of Technology, Department of Chemical Engineering, Cambridge, Massachusetts. Estados Unidos de América |
| Year: | 2004 |
| Season: | Dic |
| Volumen: | 21 |
| Number: | 4 |
| Pages: | 531-544 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Two-phase aqueous micellar systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental and theoretical work by Blankschtein and co-workers on the use of two-phase aqueous micellar systems for the separation of hydrophilic proteins. The experimental partitioning behavior of the enzyme glucose-6-phosphate dehydrogenase (G6PD) in two-phase aqueous micellar systems is also reviewed and new results are presented. Specifically, we discuss very recent work on the purification of G6PD using: i) a two-phase aqueous micellar system composed of the nonionic surfactant n-decyl tetra(ethylene oxide) (C10E4), and (ii) a two-phase aqueous mixed micellar system composed of C10E4 and the cationic surfactant decyltrimethylammonium bromide (C10TAB). Our results indicate that the two-phase aqueous mixed (C10E4/C10TAB) micellar system can improve significantly the partitioning behavior of G6PD relative to that observed in the two-phase aqueous C10E4 micellar system |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Purificación de proteínas, Sistemas micelares, Interacciones de volumen excluido, Interacciones electrostáticas |
| Keyword: | Chemistry, Medicinal chemistry, Protein purification, Micellar systems, Excluded-volume interactions, Electrostatic interactions |
| Full text: | Texto completo (Ver HTML) |
