| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308722 |
| ISSN: | 0104-6632 |
| Authors: | Falco, A.L.P1 Durrant, L.R Franco, T.T2 |
| Institutions: | 1Universidade Estadual de Campinas, Departamento de Engenharia de Alimentos, Campinas, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Departamento de Engenharia Quimica, Campinas, Sao Paulo. Brasil |
| Year: | 2000 |
| Season: | Dic |
| Volumen: | 17 |
| Number: | 4-7 |
| Pages: | 819-826 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Alpha-galactosidase taken from a raw extract of Sesbania marginata legume seeds was purified by partitioning in aqueous two-phase systems (ATPS). Initially, galactomannan/dextran 2,000,000 systems were used for the purification, and the partition coefficients of alpha -galactosidase varied from 1.5 to 4.0. However, mass transport in these systems was poor due to the high viscosity of the employed polymers. Therefore, partitioning in polyethyleneglycol (PEG)/ sodium phosphate systems and the effect of sodium chloride upon the enzyme purification and the yield of alpha -galactosidase were also investigated. The purification achieved in a single-step was 5.7 with a recovery of 144% of alpha -galactosidase, possibly due to the removal of materials which inhibited alpha -galactosidase activity before the purification. The removal of the main protein contaminants and the highest yields were achieved in PEG 4,000/ sodium phosphate + 6% NaCl system at pH 5.0. Further purification by preparative on-exchange chromatography was also developed |
| Disciplines: | Química |
| Keyword: | Química de alimentos, Semillas, Purificación de enzimas, Alfa-galactosidasa, Sesbania marginata |
| Keyword: | Chemistry, Food chemistry, Seeds, Enzyme purification, Alpha-galactosidase, Sesbania marginata |
| Full text: | Texto completo (Ver HTML) |
