| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308736 |
| ISSN: | 0104-6632 |
| Authors: | Trevisan, H.C1 Mei, L.H.I2 Zanin, G.M3 |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Quimica, Araraquara, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil 3Fundacao Universidade Estadual de Maringa, Departamento de Engenharia Quimica, Maringa, Parana. Brasil |
| Year: | 2000 |
| Season: | Mar |
| Volumen: | 17 |
| Number: | N |
| Pages: | 71-77 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A simple method for the preparation of silica with controlled pore size, for use as a support for the immobilization of enzymes, is described in this article. Using sodium silicate and hydrochloric acid, a microporous silica was obtained that was then submitted to a hydrothermal treatment, resulting in macroporous silica suitable for enzyme immobilization. Suitability of the macroporous silica as a support depends on the method chosen for its preparation, which will determine pore volume and the effect of hydrothermal treatment on pore size. The pore volume of the support was 0.8-0.9 cc/g and the average pore size, controlled by the hydrothermal treatment, was in the range of 16 to 75 nm. The enzyme amyloglucosidase was used for the immobilization studies |
| Disciplines: | Química |
| Keyword: | Ingeniería química, Biotecnología, Inmovilización enzimática, Materiales de soporte, Sílica, Amiloglucosidasa |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Enzyme immobilization, Support material, Silica, Amyloglucosidase |
| Full text: | Texto completo (Ver HTML) |
