Inulinase from Kluyveromyces marxianus: culture medium composition and enzyme extraction



Document title: Inulinase from Kluyveromyces marxianus: culture medium composition and enzyme extraction
Journal: Brazilian journal of chemical engineering
Database: PERIÓDICA
System number: 000308637
ISSN: 0104-6632
Authors: 1
Institutions: 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil
Year:
Season: Sep
Volumen: 16
Number: 3
Pages: 237-245
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract K. marxianus DSM 70106 was cultivated for inulinase production in a medium containing 2.0 g/L of yeast extract, 5.0 g/L of peptone and salts. The addition of corn steep liquor did not increase enzyme production. Inulin, as the main carbon source, afforded higher inulinase production than glucose, fructose, sucrose, lactose, maltose and starch. Glucose, fructose and sucrose reduced enzyme production by 46, 58 and 71%, respectively. By using the best culture medium enzyme activity remained stable for 22 months at 4oC; while at -18oC it decreased by 10%. Maximal activity was found in the pH range of 3.5 to 5.0 and at temperatures from 50 to 60oC. Flocculation was used for cell separation. Shifting the pH was more efficient than using polyelectrolytes, CaCl2, bentonite and Fe2O3. Recovery of inulinase by AOT(sodium di-2-ethylhexyl sulfosuccinate)-reversed micelles yielded up to ~20%
Disciplines: Química
Keyword: Química farmacéutica,
Inulinasa,
Medios de cultivo,
Extracción química,
Kluyveromyces marxianus
Keyword: Chemistry,
Medicinal chemistry,
Inulinase,
Culture media,
Chemical extraction,
Kluyveromyces marxianus
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