| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308924 |
| ISSN: | 0104-6632 |
| Authors: | Ferreira, A.L.O1 Giordano, R.L.C Giordano, R.C |
| Institutions: | 1Universidade Federal de Sao Carlos, Departamento de Engenharia Quimica, Sao Carlos, Sao Paulo. Brasil |
| Year: | 2004 |
| Season: | Dic |
| Volumen: | 21 |
| Number: | 4 |
| Pages: | 519-529 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimated using a 2(5) factorial design. A batch reactor was employed to assess the influence of the following variables: pH, temperature, initial 6-APA concentration, buffer concentration, and the presence of methanol. Response variables were productivity, selectivity, and yield (based on initial 6-APA concentration). The best synthesis yield (56.9%) was at T = 4ºC and pH 6.5. The highest productivity (49.3 ´ 10-3mM of antibiotic/min) was achieved at T = 25ºC and pH 6.5. Our results indicate that it is possible to achieve high productivity for this system while maintaining a high selectivity and yield |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Biotecnología, Enzimas inmovilizadas, Síntesis enzimática, Ampicilina, Diseño factorial |
| Keyword: | Chemistry, Medicinal chemistry, Biotechnology, Immobilized enzymes, Enzymatic synthesis, Ampicillin, Factorial design |
| Full text: | Texto completo (Ver HTML) |
