| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000309023 |
| ISSN: | 0104-6632 |
| Authors: | Alonso, F.O.M1 Oliveira, E.B.L Dellamora Ortiz, G.M Pereira-Meirelles, F.V2 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Faculdade de Farmacia, Rio de Janeiro. Brasil 2Pontificia Universidade Catolica do Rio de Janeiro, Departamento de Quimica, Rio de Janeiro. Brasil |
| Year: | 2005 |
| Season: | Mar |
| Volumen: | 22 |
| Number: | 1 |
| Pages: | 9-18 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Lipase production by a Brazilian wild strain of Yarrowia lipolytica at different stirring speeds and air flow rates was studied. The relationship among lipid consumption, cell growth and lipase production by this microorganism is presented. The most pronounced effect of oxygen on lipase production was determined by stirring speed. Maximum lipase activity was detected in the late stationary phase at 200 rpm and an air flow rate of 1-2 dm³/min (0.8-1.7 vvm) when the lipid source had been fully consumed. Higher stirring speeds resulted in mechanical and/or oxidative stress, while lower stirring speeds seemed to limit oxygen levels. An increase in the availability of oxygen at higher air flow rates led to faster lipid uptake and anticipation of enzyme release into the culture medium. The highest lipase production was obtained at 200 rpm and 1 dm³/min (0.8 vvm) |
| Disciplines: | Química |
| Keyword: | Ingeniería química, Biotecnología, Lipasas, Rendimiento, Agitación, Aereación, Yarrowia lipolytica |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Lipases, Yield, Stirring, Aeration, Yarrowia lipolytica |
| Full text: | Texto completo (Ver HTML) |
