Growth and enzyme production during continuous cultures of a high amylase-producing variant of Aspergillus oryzae
Document title: Growth and enzyme production during continuous cultures of a high amylase-producing variant of Aspergillus oryzae
Journal: Brazilian journal of chemical engineering
Database: PERIÓDICA
System number: 000308851
ISSN: 0104-6632
Authors: 1
2

3
Institutions: 1Universidade Federal de Sao Carlos, Departamento de Engenharia Quimica, Sao Carlos, Sao Paulo. Brasil
2Technical University of Denmark, Center for Process Biotechnology, Lyngby. Dinamarca
3Technical University of Denmark, Computer Aided Process Engineering Center, Lyngby, Viborg. Dinamarca
Year:
Season: Mar
Volumen: 19
Number: 1
Pages: 55-68
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Growth and product formation by a selected variant of Aspergillus oryzae showing high alpha-amylase production was studied in continuous cultivations carried out at six different specific growth rates, using glucose as the growth-limiting nutrient. The analysis of the steady-state data revealed that the variant and wild-type strains were similar with respect to glucose uptake system and stoichiometric coefficients. However, the variant was capable of maintaining an enzyme production as high as 40 FAUgDW-1h-1 at a dilution rate of 0.2 h-1, while the wild-type strain reached a maximum specific alpha-amylase production rate of 17 FAUgDW-1h-1 at a dilution rate of 0.1 h-1. Using a morphologically structured model originally proposed for the wild-type strain, it was possible to describe enzyme production, biomass formation and glucose consumption after modification of a few parameters to adjust the model to the characteristics of the selected variant
Disciplines: Química
Keyword: Biotecnología,
Ingeniería química,
Cultivo continuo,
Crecimiento,
Producción de enzimas,
Alfa amilasa,
Aspergillus oryzae
Keyword: Chemistry,
Biotechnology,
Chemical engineering,
Continuous culture,
Growth,
Enzyme production,
Alpha-amylase,
Aspergillus oryzae
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