Revista: | Brazilian journal of chemical engineering |
Base de datos: | PERIÓDICA |
Número de sistema: | 000308851 |
ISSN: | 0104-6632 |
Autores: | Zangirolami, T.C1 Carlsen, M2 Nielsen, J Jørgensen, S.B3 |
Instituciones: | 1Universidade Federal de Sao Carlos, Departamento de Engenharia Quimica, Sao Carlos, Sao Paulo. Brasil 2Technical University of Denmark, Center for Process Biotechnology, Lyngby. Dinamarca 3Technical University of Denmark, Computer Aided Process Engineering Center, Lyngby, Viborg. Dinamarca |
Año: | 2002 |
Periodo: | Mar |
Volumen: | 19 |
Número: | 1 |
Paginación: | 55-68 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Growth and product formation by a selected variant of Aspergillus oryzae showing high alpha-amylase production was studied in continuous cultivations carried out at six different specific growth rates, using glucose as the growth-limiting nutrient. The analysis of the steady-state data revealed that the variant and wild-type strains were similar with respect to glucose uptake system and stoichiometric coefficients. However, the variant was capable of maintaining an enzyme production as high as 40 FAUgDW-1h-1 at a dilution rate of 0.2 h-1, while the wild-type strain reached a maximum specific alpha-amylase production rate of 17 FAUgDW-1h-1 at a dilution rate of 0.1 h-1. Using a morphologically structured model originally proposed for the wild-type strain, it was possible to describe enzyme production, biomass formation and glucose consumption after modification of a few parameters to adjust the model to the characteristics of the selected variant |
Disciplinas: | Química |
Palabras clave: | Biotecnología, Ingeniería química, Cultivo continuo, Crecimiento, Producción de enzimas, Alfa amilasa, Aspergillus oryzae |
Keyword: | Chemistry, Biotechnology, Chemical engineering, Continuous culture, Growth, Enzyme production, Alpha-amylase, Aspergillus oryzae |
Texto completo: | Texto completo (Ver HTML) |