| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000309066 |
| ISSN: | 0104-6632 |
| Authors: | Rech, R1 Ayub, M.A.Z |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Instituto de Ciencia e Tecnologia de Alimentos, Porto Alegre, Rio Grande do Sul. Brasil |
| Year: | 2006 |
| Season: | Dic |
| Volumen: | 23 |
| Number: | 4 |
| Pages: | 435-442 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest beta-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate |
| Disciplines: | Química, Biología |
| Keyword: | Biotecnología, Levaduras recombinantes, Cultivo por lotes, Suero de queso, Beta galactosidasa, Saccharomyces cerevisiae |
| Keyword: | Chemistry, Biology, Biotechnology, Recombinant yeasts, Fed-batch culture, Cheese whey, Beta-galactosidase, Saccharomyces cerevisiae |
| Full text: | Texto completo (Ver HTML) |
