| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308934 |
| ISSN: | 0104-6632 |
| Authors: | Larentis, A. L1 Almeida, M. S Cabral, K.M.S Medeiros, L.N Kurtenbach, E Coelho, M. A. Z2 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Instituto de Ciencias Biomedicas, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Escola de Quimica, Rio de Janeiro. Brasil |
| Year: | 2004 |
| Season: | Jun |
| Volumen: | 21 |
| Number: | 2 |
| Pages: | 155-164 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The methylotrophic yeast Pichia pastoris was used to produce the recombinant Pisum sativum defensin (rPsd1), a small peptide from pea seeds that has a high level of antifungal activity. The plasmid rPsd1/pPIC9 was integrated into the yeast genome and methanol was used to induce expression and secretion of the recombinant Psd1, at 30º C in a fed-batch mode. The effects of different pH conditions and process scale-up were evaluated using a Monod-type model where dissolved oxygen was considered the limiting substrate. Parameter estimation showed that the process could be improved by expressing rPsd1 in a 1000 mL bioreactor at pH 4. Structural and functional analyses revealed that the recombinant Psd1 is very similar to the native one |
| Disciplines: | Química |
| Keyword: | Bioquímica, Biotecnología, Cultivo por lotes, Pichia pastoris, Pisum sativum, Expresión génica, pH, Defensinas, ADN recombinante |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Fed-batch culture, Pichia pastoris, Pisum sativum, Gene expression, pH, Defensins, Recombinant DNA |
| Full text: | Texto completo (Ver HTML) |
