| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308996 |
| ISSN: | 0104-6632 |
| Authors: | Neto, A.B1 Hirata, D.B Cassiano-Filho, L.C.M Bellao, C Badino-Junior, A.C Hokka, C.O |
| Institutions: | 1Universidade Federal de Sao Carlos, Departamento de Engenharia Quimica, Sao Carlos, Sao Paulo. Brasil |
| Year: | 2005 |
| Season: | Dic |
| Volumen: | 22 |
| Number: | 4 |
| Pages: | 557-563 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Clavulanic acid (CA) is a potent inhibitor of beta-lactamases, enzymes that are responsible for the hydrolysis of beta-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasing productivity. The operating conditions: temperature, aeration and agitation, were the same in all cases, 28º C, 0.5 vvm and 800 rpm, respectively. The CA concentration obtained in the fed-batch culture, 404 mg L-1, was ca twice the value obtained in the batch culture, 194 mg L-1, while 293 mg L-1 was obtained in the continuous culture. The highest productivity was obtained in the continuous cultivation, 10.6 mg L-1 h-1, as compared with 8.8 mg L-1 h-1 in the fed-batch process and 3.5 mg L-1 h-1 in the batch process, suggesting that continuous culture of Streptomyces clavuligerus is a promising strategy for clavulanic acid production |
| Disciplines: | Química |
| Keyword: | Ingeniería química, Biotecnología, Streptomyces clavuligerus, Biorreactores, Proceso por lotes, Proceso continuo, Acido clavulánico |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Streptomyces clavuligerus, Bioreactors, Batch process, Continuous process, Clavulanic acid |
| Full text: | Texto completo (Ver HTML) |
