| Journal: | Brazilian dental journal |
| Database: | PERIÓDICA |
| System number: | 000290303 |
| ISSN: | 0103-6440 |
| Authors: | Sousa, Simone Maria Galvao de1 Bramante, Clovis Monteiro2 Taga, Eulazio Mikio |
| Institutions: | 1Pontificia Universidade Catolica do Rio de Janeiro, Departamento de Ciencias en Materiales y Metalurgia, Rio de Janeiro. Brasil 2Universidade de Sao Paulo, Faculdade de Odontologia, Bauru, Sao Paulo. Brasil |
| Year: | 2005 |
| Season: | Ene-Abr |
| Volumen: | 16 |
| Number: | 1 |
| Pages: | 3-8 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | This in vivo study evaluated, through the physicochemical assay method for quantification of enhanced vascular permeability, the irritating potential of EDTA, EGTA, citric acid and saline. Thirty-two male Wister rats were anesthetized and four experimental sites were demarcated on their backs. Injections of 2% Evans blue (20 mg/kg) were administered intravenously into the lateral caudal vein. The test solutions were immediately injected intradermally (0.01 mL) into the experimental sites. The animals were killed 30 min, 1, 3 and 6 h after injection of the solutions and each piece of skin was submerged in formamide and incubated at 45ºC for 72 h. After filtration, the optical density was measured in a spectrophotometer and the total amount of dye extracted from the samples was calculated by means of a standard calibration curve. Data were analyzed statistically by two-way ANOVA and Tukey's HSD test. Compared to control, EDTA had the greatest volume of dye followed by EGTA and citric acid, for all time periods. There were statistically significant differences between all solutions (p<0.01). Considering the periods assessed, a significant difference was observed between the 3- and 6-h groups (p<0.05), but not between the 30-min and 1-h groups. Among the organic acids evaluated in this study, citric acid yielded the lowest amount of extracted dye. This indicates that the citric acid was the least irritating solution |
| Portuguese abstract | Este estudo in vivo avaliou o potencial irritativo do EDTA, EGTA, ácido cítrico e soro fisiológico (controle) durante a fase exsudativa do processo inflamatório. Aplicou-se, intravenosamente na veia caudal lateral de 32 ratos machos da linhagem "Wistar", variação albina, 20 mg/kg de azul de Evans 2%. Em seguida, no tecido subcutâneo da região dorsal dos animais injetou-se 0,01 mL das soluções testes. Após os intervalos de ½, 1, 3 e 6 horas, os animais foram sacrificados, suas peles dorsais foram excisadas e submetidas à análise do corante extravasado pela espectrofotometria de absorção de luz. Os dados obtidos foram avaliados pela análise de variância a 2 critérios e teste de Tukey. Em todos os períodos de tempo estudados, os maiores valores de corante extravasado foram observados no grupo do EDTA seguido pelos grupos do EGTA e ácido cítrico, em comparação ao grupo controle. Houve diferença estatisticamente significante entre todas as soluções testadas (p<0.01). Quando considerado o fator tempo, notou-se diferença significante entre os grupos de 3 e 6 horas (p<0.05). Entretanto, não houve diferença entre os grupos de tempo de ½ e 1 hora. Dentre os ácidos orgânicos avaliados, os resultados demonstraram que o ácido cítrico apresentou o menor potencial irritativo |
| Disciplines: | Medicina |
| Keyword: | Odontología, Biocompatibilidad, Acido cítrico, EDTA, Terapia, Canal radicular |
| Keyword: | Medicine, Dentistry, Biocompatibility, Citric acid, EDTA, Therapy, Root canal |
| Full text: | Texto completo (Ver HTML) |
