Sperm Quality and Testicular Histomorphometry of Wistar Rats Supplemented with Extract and Fractions of Fruit of Tribulus terrestris L



Document title: Sperm Quality and Testicular Histomorphometry of Wistar Rats Supplemented with Extract and Fractions of Fruit of Tribulus terrestris L
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392842
ISSN: 1516-8913
Authors: 1
2
2
2
2
2
1
1
2
Institutions: 1Universidade Federal de Lavras, Departamento de Agricultura, Lavras, Minas Gerais. Brasil
2Universidade Federal de Lavras, Departamento de Medicina Veterinaria, Lavras, Minas Gerais. Brasil
Year:
Season: Nov-Dic
Volumen: 58
Number: 6
Pages: 891-897
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract The aim of this study was to assess the sperm quality and testicular histomorphometry of Wistar rats supplemented with extract and fractions of fruits of Tribulus terrestris L. The ethanolic extract was obtain ed by dynamic maceration of spray - dried fruit. This extract was fractionated by liquid - liquid partition, using increasing polarity solvents. Twenty male rats were separated in four groups, with five rats in each group. The control was supplemented with dis tilled water, while the others were daily given the ethanolic extract, hexanic or aqueous fraction soluble in methanol in a dose of 42 mg.kg - 1 .day - 1 for 70 days. Sperm was obtained from the right epididymal tail for the analysis of motility, count, morphol ogy and viability. The testicular weight of groups supplemented with ethanolic extract and aqueous fraction soluble in methanol was higher when compared to the control. The gonadosomatic index increased in the group supplemented with ethanolic extract. The nuclear, cytoplasmic and individual volume of Leydig cells increased in supplementation with hexanic and aqueous fractions soluble in methanol. It was concluded that the extract influenced the spermatogenesis , while hexanic and aqueous fractions soluble i n methanol promoted the changes in the intertubular compartment. Therefore, Tribulus terrestris did not improve the sperm quality of the rats
Disciplines: Medicina veterinaria y zootecnia,
Química
Keyword: Reproducción y genética animal,
Fitoquímica,
Biología celular,
Extractos vegetales,
Espermatogénesis,
Células de Leydig,
Ratas,
Suplementación
Keyword: Veterinary medicine and animal husbandry,
Chemistry,
Animal reproduction and genetics,
Phytochemistry,
Cell biology,
Vegetal extracts,
Spermatogenesis,
Leydig cells,
Rats,
Supplementation
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