| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392775 |
| ISSN: | 1516-8913 |
| Authors: | Zhang, Yu1 Zheng, Wei1 Gu, Jian-Fei1 Ni, Jian1 Wang, Ling1 Tang, Zhen-Xing2 Shi, Lu-E1 |
| Institutions: | 1Hangzhou Normal University, College of Life and Environmental Sciences, Hangzhou, Zhejiang. China 2Nanjing Forestry University, College of Light Industry Science and Engineering, Nanjing, Jiangsu. China |
| Year: | 2015 |
| Season: | Sep-Oct |
| Volumen: | 58 |
| Number: | 5 |
| Pages: | 805-811 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | In this work, the mixture of alginate and soy protein isolate used as a wall material was developed to encapsulate Enterococcus faecalis HZNU P2 ( E. faecal is HZNU P2). The s urvival ability in the simulated gastric fluid (SGF) and bile salt solution, storage stability at different temperatures and release properties in the simulated intestinal fluid (SIF) of encapsulated cells were assessed. The results showed that encapsulation could offer sufficient protection to E. faecalis HZNU P2 . The v iability of encapsulated E. faecalis HZNU P2 did not decrease in SGF at pH 2.5 or 2.0 after 2 h incubation , while free cells were reduced from 11 to 9.85 l og CFU/mL in SGF ( pH 2.5 ) at the same exposure time . O nly minor viability of encapsulated E. faecalis HZNU P2 lost in 1 .0 or 2 .0 % bile salt solution for 1 or 2 h exposure, compared with no survival of free E. faecalis HZNU P2 under the same conditions. Encapsulated E. faec alis HZNU P2 was completely released from the microspheres in SIF within 1 h. The viability of encapsulated E. faecalis HZNU P2 stored for two weeks at 4 °C was fully retained . Viabilities of encapsulated E. faecalis HZNU P2, 9.6 and 9.0 Log CFU/g were obtained at 25 and 37 °C after 21 days storage, respectively. However, around 1.0 log CFU/mL of free cells was reduced after two weeks storage at 4 °C. Encapsulated E. faecalis HZNU P2 using soy protein isolate and alginate as wall materials could play an import ant role in food applications |
| Disciplines: | Biología, Química, Agrociencias |
| Keyword: | Bacterias, Bioquímica, Plantas para uso industrial, Soya, Enterococcus faecalis, Encapsulación, Proteínas, Alginatos, Estabilidad, Viabilidad |
| Keyword: | Biology, Chemistry, Agricultural sciences, Bacteria, Biochemistry, Plants for industrial use, Soybean, Enterococcus faecalis, Encapsulation, Proteins, Alginate, Stability, Viability |
| Full text: | Texto completo (Ver PDF) |
