| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392748 |
| ISSN: | 1516-8913 |
| Authors: | Al-Rasheed, Nouf Mohamed1 Fadda, Laila Mohamed1 Al-Rasheed, Nawal Mohamed1 Attia, Hala2 Ali, Hanaa Mahmoud3 El-Agami, Hanaa1 |
| Institutions: | 1King Saud University, Pharmacology Department, Riyadh. Arabia Saudita 2Mansoura University, Department of Biochemistry, Mansoura. Egipto 3National Research Center, El Tabir, Cairo. Egipto |
| Year: | 2015 |
| Season: | Sep-Oct |
| Volumen: | 58 |
| Number: | 5 |
| Pages: | 741-749 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The aim of this work was to investigate whether the free radical scavengers, L - arginine (L - arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer’s ratio [concentrations of branch ed chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer’s ratios and i ncreased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L - arg, ( 200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia , urea , BCAAs (valine, leucine and isoleucine) and aromatic amino acids (phenylalanine and tyrosine ). The Fischer’s ratio was decreased compared with the control; the administration of the aforementioned antioxidants ameliorated most of the previously altered p arameters. It was concluded that Fischer's ratio was a valuable tool for understanding the pathology of hemic hypoxia, evaluating the degree of the modulatory effect of various natural antioxidants and the synergy between L - arg and carnosine in amelioratin g the effect of sodium nitrite on amino acids pattern. Thus, it could be recommended to administer the combination of L - arg and carnosine in the areas of high altitudes to combat the hazard effect of hypoxia on hemoglobin concentration and MDA level |
| Disciplines: | Química, Medicina |
| Keyword: | Bioquímica, Química de alimentos, Medicina experimental, Antioxidantes, Aminoácidos, Carnosina, Urea |
| Keyword: | Chemistry, Medicine, Biochemistry, Food chemistry, Experimental medicine, Antioxidants, Amino acids, Carnosine, Urea |
| Full text: | Texto completo (Ver PDF) |
